16s rrna sequencing results  (ATCC)

Journal: ASM Case Reports

Article Title: Mycobacterium wolinskyi as an emerging cause of pacemaker pocket infection and lead endocarditis: a case report and genomic characterization

doi: 10.1128/asmcr.00146-25

Figure Lengend Snippet: Phylogenetic analysis based on 16S rRNA ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).

Article Snippet: Because of discrepant 16S rRNA sequencing results and low ANI percentage compared with the M. wolinskyi reference genome (strain ATCC 700010), a phylogenetic analysis was performed.

Techniques: Sequencing, Software

Journal: Frontiers in Microbiology

Article Title: Comparative evaluation of sequencing technologies and primer sets for mouse gut microbiota profiling

doi: 10.3389/fmicb.2025.1584359

Figure Lengend Snippet: 16S rRNA gene with primer binding sites used in this study.

Article Snippet: Previous studies have primarily focused on comparing 16S rRNA sequencing results between Illumina and ONT, assessing taxonomic resolution and rare taxa detection while largely overlooking MS data.

Techniques: Binding Assay

Journal: Frontiers in Microbiology

Article Title: Comparative evaluation of sequencing technologies and primer sets for mouse gut microbiota profiling

doi: 10.3389/fmicb.2025.1584359

Figure Lengend Snippet: Microbial community analysis in experimental mice via full-length 16S rRNA gene sequencing. (A) Raincloud plot of the alpha diversity index. Primer pairs are displayed along the x-axis, with the Shannon index represented on the y-axis, color-coded by primer. (B) PCoA plot illustrating microbial community composition. Each point represents a sample, plotted using Bray-Curtis distances, with colors indicating group classifications. The distinct cluster on the left corresponds to baseline microbiota profiles collected before bacterial culture administration. (C) UpSet plot showing unique and shared bacterial species across primer sets. Bars indicate the number of species detected by each primer combination.

Article Snippet: Previous studies have primarily focused on comparing 16S rRNA sequencing results between Illumina and ONT, assessing taxonomic resolution and rare taxa detection while largely overlooking MS data.

Techniques: Sequencing

Journal: Microorganisms

Article Title: Nanopore Is Preferable over Illumina for 16S Amplicon Sequencing of the Gut Microbiota When Species-Level Taxonomic Classification, Accurate Estimation of Richness, or Focus on Rare Taxa Is Required

doi: 10.3390/microorganisms11030804

Figure Lengend Snippet: Published studies comparing 16S rRNA gene amplicon sequencing with Illumina and Nanopore.

Article Snippet: Therefore, using faecal samples collected from six individuals and a mock community sample, we compared 16S rRNA gene amplicon sequencing results from Nanopore and Illumina to assess their ability to distinguish samples based on the bacterial community structure at species and higher taxonomic levels, deliver replicable results and good coverage of species richness.

Techniques: Amplification, Sequencing, Nanopore Sequencing, Illumina Sequencing, Comparison